Cell culturing

An organ-on-chip is a biochip for cell culturing. In biotechnology, the creation of cell cultures is an important element. This applies to bioreactors that produce biological substances via cells and also to systems in cell culturing that are used, for example, as test systems for the effect of pharmaceuticals

The culturing of whole cell lines is intended to provide comparable cell material for investigations. In order for the cells to fulfill their tasks, they must be appropriately supplied in the bioreactors. A functional organ-on-a-chip in cell culturing includes the appropriate temperature and light conditions, as well as the supply of nutrients (e.g. glucose) or the addition of growth promoters or inhibitors

Many tasks in cell culturing can be performed by automatic pump systems, which are superior to purely manual dosing in terms of quality and convenience. Our mp6 micropumps can be placed very close to the reactors due to their small size, low power requirements and long lifetime. This also makes placement in the incubator easy to set up.

The application of our customer C-Cit is particularly exciting here: A preassembled flow cell with glucose and/or lactate sensor.

About C-CIT Sensors AG

C-Cit Sensors AG has developed a system that can measure glucose and/or lactates in a flow cell. This organ-on-a-chip is particularly useful in checking cell growth.

An organ-on-a-chip is a multi-channel 3-D microfluidic biochip that simulates the activities, mechanics and physiological response of whole organs and organ systems – a kind of artificial organ.

The flow cell is the best choice for long-term monitoring in tube-based reactor systems as well as in any perfusion-based bioprocess. It features a flat bottom with no dead volume, allowing the culture medium to flow through the cell and avoiding cell aggregation in or around the sensor.

The flow cell housing and cover are made of USP VI certified material and the flow cell is gamma sterilized. Connected to the C-Cit systems CITSens Bio or CITSens MeMo and the mp6 micropump, the flow cell can be used for media delivery. It can be connected with either Luerlock or weldable tubing and can also be ordered as a pre-assembled system with an mp6 micropump.

The mp6 micropump handles reagent delivery to the sensor in this cell culturing application. By using a micropump, the entire organ-on-a-chip application remains very small and only small amounts of reagents are required.